Development and standardization of a cryptosporidium genotyping tool for water samples
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Development and standardization of a cryptosporidium genotyping tool for water samples

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Published by AWWA Research Foundation/American Water Works Association/IWA Pub. in Denver, CO .
Written in English

Subjects:

  • Water -- Sampling -- Standards.,
  • Cryptosporidium -- Genetics.,
  • Drinking water -- Contamination.,
  • Cryptosporidium -- Control.,
  • DNA -- Databases.

Book details:

Edition Notes

Statementprepared by Lihua Xiao, Kerri Alderisio, and Ajaib Singh.
ContributionsAlderisio, Kerri., Singh, Ajaib., AWWA Research Foundation., American Water Works Association., IWA Publishing.
Classifications
LC ClassificationsTD427.C78 X53 2006
The Physical Object
Paginationxxii, 116 p. :
Number of Pages116
ID Numbers
Open LibraryOL19708577M
ISBN 101583214410
ISBN 109781583214411
LC Control Number2006049852

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Development and Standardization of a Cryptosporidium Genotyping Tool for Water Samples Completed Summarizes and evaluates existing progress in the application of molecular techniques to Cryptosporidium analysis. Development and Standardization of a Cryptosporidium Genotyping Tool for Water Samples by American Water Works Association 1 edition - first published in Not in Library.   Cryptosporidium, faecal indicator organisms and physical and chemical water quality variables were monitored in a small mixed rural–urban watershed in southeastern sporidium was present in 43% of water samples analysed by microscopy. Concentrations varied from non-detects to 14 oocysts L − samples were further analysed by nested-PCR, and Cryptosporidium Author: Rosane C. Andrade, Rafael K. X. Bastos, Paula D. Bevilacqua, Rosângela V. Andrade.   Xiao L, Alderisio K, Singh A () Development and standardization of a Cryptosporidium genotyping tool for water samples. Awwa Research Foundation, Denver Google Scholar Yang W, Chen P, Villegas EN, Landy RB, Kanetsky C, Cama V, Dearen T, Schultz CL, Orndorff KG, Prelewicz GJ, Brown MH, Young KR, Xiao L () Cryptosporidium source tracking Cited by: 4.

City water supply system were detected and genotyped by the same rRNA-based genotyping tool previously used in the anal-ysis of storm water samples (11, 19, 20). The objectives were (i) to assess the role of wildlife in Cryptosporidium contamination in the NYCDEP watershed and to identify the remaining ge-notypes that could not be tracked to Cited by: species/genotypes was successful from of the samples analyzed. Species considered to be of high risk to humans through waterborne routes of transmission (C. hominis and C. parvum) were present in % of Cryptosporidium microscope positive samples, resulting in an overall frequency of % (based on the total number of water samples. We undertook a 1-year survey to identify the species and genotypes of Cryptosporidium oocysts detected in the Scottish Water (SW) Routine Cryptosporidium Monitoring Programme to gain information on the occurrence and diversity of Cryptosporidium oocysts in drinking water sources and drinking waters in order to determine predominant types in water catchment areas and monitor Cited by: Seventy percent of water samples positive for Cryptosporidium oocysts by immunofluorescent microscopy tested positive by molecular assays and resulted in species/genotype : Jan Slapeta.

  Johnson DW, Pieniazek NJ, Griffin DW, Misener L, Rose JB. Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Appl Environ Microbiol ; Antibody detection: There are currently no commercially available serologic assays for the detection of Cryptosporidium-specific antibodies. However. The detection of Cryptosporidium parvum oocysts in environmental water samples and the determination of oocyst infectivity are imperative issues for the water industry. An integrated Cell Culture Polymerase Chain Reaction (CC-PCR) method has been developed recently for the detection and molecular genotyping of infectious waterborne C. parvum. Evaluation of Cryptosporidium parvum Genotyping Techniques Article (PDF Available) in Applied and Environmental Microbiology 65(10) November with 74 Reads How we measure 'reads'. Cryptosporidiumdetection and genotyping. After washing the specimens twice in distilled water, genomic DNA was extracted from ml of specimens using a FastDNA SPIN kit for soil (BIO , Carlsbad, CA) and eluted in μl of reagent-grade water as described previously (10).Cited by: